Publications

IL-10 signaling in dendritic cells is required for tolerance induction in a murine model of allergic airway inflammation.

Allergen specific tolerance induction efficiently ameliorates subsequent allergen induced inflammatory responses. The underlying …

Permeability analyses and three dimensional imaging of interferon gamma- induced barrier disintegration in intestinal organoids.

The aberrant regulation of the epithelial barrier integrity is involved in many diseases of the digestive tract, including inflammatory …

Human TNF-Luc reporter mouse: A new model to quantify inflammatory responses.

Tumour necrosis factor (TNF) is a key cytokine during inflammatory responses and its dysregulation is detrimental in many inflammatory …

Innate Sensing through Mesenchymal TLR4/MyD88 Signals Promotes Spontaneous Intestinal Tumorigenesis.

MyD88, an adaptor molecule downstream of innate pathways, plays a significant tumor-promoting role in sporadic intestinal …

Macrophage-Specific NF-kappaB Activation Dynamics Can Segregate Inflammatory Bowel Disease Patients.

The heterogeneous nature of inflammatory bowel disease (IBD) presents challenges, particularly when choosing therapy. Activation of the …

Evaluating the IgMi mouse as a novel tool to study B-cell biology.

The IgMi mouse fails to secrete antibodies or class switch its BCR from IgM. Our study reveals that other cellular compartments, …

Ribonucleotide Excision Repair Is Essential to Prevent Squamous Cell Carcinoma of the Skin.

Because of imperfect discrimination against ribonucleoside triphosphates by the replicative DNA polymerases, large numbers of …

Interleukin-1beta has atheroprotective effects in advanced atherosclerotic lesions of mice.

Despite decades of research, our understanding of the processes controlling late-stage atherosclerotic plaque stability remains poor. A …

Although Abundant in Tumor Tissue, Mast Cells Have No Effect on Immunological Micro-milieu or Growth of HPV-Induced or Transplanted Tumors.

High numbers of mast cells populate the stroma of many types of neoplasms, including human papilloma virus-induced benign and malignant …

Unimpaired Responses to Vaccination With Protein Antigen Plus Adjuvant in Mice With Kit-Independent Mast Cell Deficiency.

Innate inflammatory responses are crucial for induction and regulation of T cell and antibody responses. Mast cell (MC)-deficient Kit …

IL-6-Type Cytokine Signaling in Adipocytes Induces Intestinal GLP-1 Secretion.

We recently showed that interleukin (IL)-6-type cytokine signaling in adipocytes induces free fatty acid release from visceral …

Involvement of interleukin-1 type 1 receptors in lipopolysaccharide- induced sickness responses.

Sickness responses to lipopolysaccharide (LPS) were examined in mice with deletion of the interleukin (IL)-1 type 1 receptor (IL-1R1). …

Gamma Interferon Mediates Experimental Cerebral Malaria by Signaling within Both the Hematopoietic and Nonhematopoietic Compartments.

Experimental cerebral malaria (ECM) is a gamma interferon (IFN- gamma)-dependent syndrome. However, whether IFN-gamma promotes ECM …

Guidelines for the use of flow cytometry and cell sorting in immunological studies.

TGF-beta inhibitor Smad7 regulates dendritic cell-induced autoimmunity.

TGF-beta is an anti-inflammatory cytokine whose signaling is negatively controlled by Smad7. Previously, we established a role for …

P2X7 receptor-dependent tuning of gut epithelial responses to infection.

Infection and injury of the gut are associated with cell damage and release of molecules such as extracellular adenosine …

IL-1 signaling is critical for expansion but not generation of autoreactive GM-CSF+ Th17 cells.

Interleukin-1 (IL-1) is implicated in numerous pathologies, including multiple sclerosis and its animal model experimental autoimmune …

Constitutive Kit activity triggers B-cell acute lymphoblastic leukemia- like disease in mice.

Acute lymphoblastic leukemia (ALL) is the most common childhood malignancy and, in most cases, is of pro- or pre-B cell origin (B-ALL). …

CD4(+) Th2 cells are directly regulated by IL-10 during allergic airway inflammation.

Interleukin-10 (IL-10) is an important regulatory cytokine required to control allergy and asthma. IL-10-mediated regulation of T …

Loss of Trex1 in Dendritic Cells Is Sufficient To Trigger Systemic Autoimmunity.

Defects of the intracellular enzyme 3’ repair exonuclease 1 (Trex1) cause the rare autoimmune condition Aicardi-Goutieres …

T cell derived IL-10 is dispensable for tolerance induction in a murine model of allergic airway inflammation.

Regulatory mechanisms initiated by allergen-specific immunotherapy are mainly attributed to T cell derived IL-10. However, it has not …

Characterization of a conditional interleukin-1 receptor 1 mouse mutant using the Cre/LoxP system.

IL-1 is a key cytokine known to drive chronic inflammation and to regulate many physiological, immunological, and neuroimmunological …

Myeloid interferon-gamma receptor deficiency does not affect atherosclerosis in LDLR(-/-) mice.

BACKGROUND AND AIMS: Atherosclerosis is a chronic lipid-driven inflammatory disease of the arterial wall. Interferon gamma (IFNgamma) …

Colonic gene silencing using siRNA-loaded calcium phosphate/PLGA nanoparticles ameliorates intestinal inflammation in vivo.

Cytokines and chemokines are predominant players in the progression of inflammatory bowel diseases. While systemic neutralization of …

Mesenteric Fat Lipolysis Mediates Obesity-Associated Hepatic Steatosis and Insulin Resistance.

Hepatic steatosis and insulin resistance are among the most prevalent metabolic disorders and are tightly associated with obesity and …

Genetic Cell Ablation Reveals Clusters of Local Self-Renewing Microglia in the Mammalian Central Nervous System.

During early embryogenesis, microglia arise from yolk sac progenitors that populate the developing central nervous system (CNS), but …

Analysis of mammalian gene function through broad-based phenotypic screens across a consortium of mouse clinics.

The function of the majority of genes in the mouse and human genomes remains unknown. The mouse embryonic stem cell knockout resource …

Regulatory T cells and T-cell-derived IL-10 interfere with effective anti-cytomegalovirus immune response.

Cytomegalovirus (CMV) establishes lifelong chronic infection in its host with mostly asymptomatic or only mild disease, but under …

Glycoprotein 130 receptor signaling mediates alpha-cell dysfunction in a rodent model of type 2 diabetes.

Dysregulated glucagon secretion accompanies islet inflammation in type 2 diabetes. We recently discovered that interleukin (IL)-6 …

Transient ablation of regulatory T cells improves antitumor immunity in colitis-associated colon cancer.

Regulatory T cells (Treg) are supportive to cancer development in most tissues, but their role in colitis-associated colon cancer (CAC) …

Protective mucosal immunity mediated by epithelial CD1d and IL-10.

The mechanisms by which mucosal homeostasis is maintained are of central importance to inflammatory bowel disease. Critical to these …

Macrophage-restricted interleukin-10 receptor deficiency, but not IL-10 deficiency, causes severe spontaneous colitis.

Interleukin-10 (IL-10) is a pleiotropic anti-inflammatory cytokine produced and sensed by most hematopoietic cells. Genome-wide …

IFN-gamma-mediated induction of an apical IL-10 receptor on polarized intestinal epithelia.

Cytokines secreted at sites of inflammation impact the onset, progression, and resolution of inflammation. In this article, we …

Monocyte-derived dendritic cells perform hemophagocytosis to fine-tune excessive immune responses.

Because immune responses simultaneously defend and injure the host, the immune system must be finely regulated to ensure the host's …

Interferon-dependent IL-10 production by Tregs limits tumor Th17 inflammation.

The capacity of IL-10 and Tregs in the inflammatory tumor microenvironment to impair anticancer Th1 immunity makes them attractive …

TGF-beta signalling is required for CD4(+) T cell homeostasis but dispensable for regulatory T cell function.

TGF-beta is widely held to be critical for the maintenance and function of regulatory T (T(reg)) cells and thus peripheral tolerance. …

T-cell-derived, but not B-cell-derived, IL-10 suppresses antigen-specific T-cell responses in Litomosoides sigmodontis-infected mice.

IL-10, a cytokine with pleiotropic functions is produced by many different cells. Although IL-10 may be crucial for initiating …

T cell-derived IL-10 determines leishmaniasis disease outcome and is suppressed by a dendritic cell based vaccine.

In the murine model of Leishmania major infection, resistance or susceptibility to the parasite has been associated with the …

CD4+ T cell-derived IL-10 promotes Brucella abortus persistence via modulation of macrophage function.

Evasion of host immune responses is a prerequisite for chronic bacterial diseases; however, the underlying mechanisms are not fully …

IL-10 acts as a developmental switch guiding monocyte differentiation to macrophages during a murine peritoneal infection.

The peritoneal wash of BALB/c or C57BL/6 mice contains two populations of macrophages that differ in their level of expression of MHC …

beta7 integrin controls immunogenic and tolerogenic mucosal B cell responses.

IgA production in the gut-associated lymphoid tissue represents a pivotal defense mechanism against luminal pathogens. The other …

Neuronal gp130 expression is crucial to prevent neuronal loss, hyperinflammation, and lethal course of murine Toxoplasma encephalitis.

The obligate intracellular parasite Toxoplasma gondii infects and persists within neurons of approximately one-third of the human …

Site-specific immunophenotyping of keloid disease demonstrates immune upregulation and the presence of lymphoid aggregates.

BACKGROUND: Keloid disease (KD) is a common fibroproliferative disorder of unknown aetiology. T cells and macrophages are increased in …

Induction of regulatory T cells by a murine beta-defensin.

beta-Defensins are antimicrobial peptides of the innate immune system produced in the skin by various stimuli, including …

B cell-derived IL-10 does not regulate spontaneous systemic autoimmunity in MRL.Fas(lpr) mice.

B cells contribute to the pathogenesis of chronic autoimmune disorders, like systemic lupus erythematosus (SLE), via multiple effector …

Commensal gut flora reduces susceptibility to experimentally induced colitis via T-cell-derived interleukin-10.

BACKGROUND: Regulatory cytokines are well known to modify experimental colitis in mice. The aim of this study was to elucidate the …

Autocrine regulation of pulmonary inflammation by effector T-cell derived IL-10 during infection with respiratory syncytial virus.

Respiratory syncytial virus (RSV) infection is the leading viral cause of severe lower respiratory tract illness in young infants. …

gp130 on macrophages/granulocytes modulates inflammation during experimental tuberculosis.

gp130 is a common receptor chain for cytokines such as interleukin (IL)-27 and IL-6. During experimental tuberculosis (TB), IL-27 …

Pro-B cells sense productive immunoglobulin heavy chain rearrangement irrespective of polypeptide production.

B-lymphocyte development is dictated by the protein products of functionally rearranged Ig heavy (H) and light (L) chain genes. Ig …

Mast cells are key promoters of contact allergy that mediate the adjuvant effects of haptens.

A prominent feature of sensitizing environmental compounds that cause allergic contact dermatitis is the rapid induction of an innate …

Gp130-dependent astrocytic survival is critical for the control of autoimmune central nervous system inflammation.

Astrocytes are activated in experimental autoimmune encephalomyelitis (EAE) and have been suggested to either aggravate or ameliorate …

Gp130-dependent release of acute phase proteins is linked to the activation of innate immune signaling pathways.

BACKGROUND: Elevated levels of acute phase proteins (APP) are often found in patients with cardiovascular diseases. In a previous …

Interleukin-10 signaling in regulatory T cells is required for suppression of Th17 cell-mediated inflammation.

Effector CD4+ T cell subsets, whose differentiation is facilitated by distinct cytokine cues, amplify the corresponding type of …

Intestinal tolerance requires gut homing and expansion of FoxP3+ regulatory T cells in the lamina propria.

Tolerance to food antigen manifests in the absence and/or suppression of antigen-specific immune responses locally in the gut but also …

TLR-2-activated B cells suppress Helicobacter-induced preneoplastic gastric immunopathology by inducing T regulatory-1 cells.

B cells regulate autoimmune pathologies and chronic inflammatory conditions such as autoimmune encephalomyelitis and inflammatory bowel …

Transgenic mice with a diverse human T cell antigen receptor repertoire.

Because of tolerance mechanisms, it has been hard to identify the T cell receptors (TCRs) of high-avidity T cells against self (for …

Differential roles of macrophages in diverse phases of skin repair.

Influx of macrophages plays a crucial role in tissue repair. However, the precise function of macrophages during the healing response …

Monocytes/macrophages and/or neutrophils are the target of IL-10 in the LPS endotoxemia model.

IL-10 is a potent regulator of the innate and adaptive immune responses. Several cell types produce IL-10 and its receptor chains and …

EuroPhenome: a repository for high-throughput mouse phenotyping data.

The broad aim of biomedical science in the postgenomic era is to link genomic and phenotype information to allow deeper understanding …

Preconditioning-induced protection of photoreceptors requires activation of the signal-transducing receptor gp130 in photoreceptors.

Retinal degenerations are a class of neurodegenerative disorders that ultimately lead to blindness due to the death of retinal …

Hepatocyte gp130 deficiency reduces vascular remodeling after carotid artery ligation.

Inflammation and vascular remodeling are hallmarks of atherosclerosis, hypertension, and restenosis after angioplasty. Here we …

Langerhans cells suppress contact hypersensitivity responses via cognate CD4 interaction and langerhans cell-derived IL-10.

Mice lacking epidermal Langerhans cells (LC) develop exaggerated contact- hypersensitivity (CHS) responses due to the absence of LC …

Charles River altered Schaedler flora (CRASF) remained stable for four years in a mouse colony housed in individually ventilated cages.

As recommendations for specific pathogen-free housing change, mouse facilities need to re-derive their colonies repeatedly in order to …

Mucosal addressin cell-adhesion molecule-1 controls plasma-cell migration and function in the small intestine of mice.

BACKGROUND & AIMS: Immunoglobulin (Ig) A secretion into the intestinal lumen is an important immune mechanism of the …

Nonredundant roles for B cell-derived IL-10 in immune counter-regulation.

IL-10 plays a central role in restraining the vigor of inflammatory responses, but the critical cellular sources of this …

T-cell-specific deletion of gp130 renders the highly susceptible IL-10-deficient mouse resistant to intestinal nematode infection.

Gp130 is the common receptor of the IL-6 family of cytokines and is involved in many biological processes, including acute phase …

Functional knockdown of VCAM-1 at the posttranslational level with ER retained antibodies.

Vascular cell adhesion molecule 1 (VCAM-1) is involved in the recruitment of leukocytes to inflammatory sites. In this study we present …

Conditional deletion of the MHC class I-related receptor FcRn reveals the sites of IgG homeostasis in mice.

The MHC class I-related receptor FcRn regulates the levels and persistence of IgG in vivo. This receptor salvages IgG from lysosomal …

A key role for gp130 expressed on peripheral sensory nerves in pathological pain.

Interleukin-6 (IL-6) is a key mediator of inflammation. Inhibitors of IL-6 or of its signal transducing receptor gp130 constitute a …

IL-20 receptor 2 signaling down-regulates antigen-specific T cell responses.

The recently described cytokines IL-19, IL-20, and IL-24 share structural homology with IL-10 and are therefore classified as members …

Excessive CpG 1668 stimulation triggers IL-10 production by cDC that inhibits IFN-alpha responses by pDC.

Upon stimulation with a wide range of concentrations of CpG oligodeoxynucleotide 2216 (CpG 2216), plasmacytoid DC are induced to …

Tolerance without clonal expansion: self-antigen-expressing B cells program self-reactive T cells for future deletion.

B cells have been shown in various animal models to induce immunological tolerance leading to reduced immune responses and protection …

Astrocyte gp130 expression is critical for the control of Toxoplasma encephalitis.

Toxoplasma gondii infects astrocytes, neurons and microglia cells in the CNS and, after acute encephalitis, persists within neurons. …

Conditional gp130 deficient mouse mutants.

The common cytokine receptor chain, gp130, controls the activity of a group of cytokines, namely, IL-6, IL-11, IL-27, ciliary …

Molecular mimicry between neurons and an intracerebral pathogen induces a CD8 T cell-mediated autoimmune disease.

To identify basic mechanisms of how infections may induce a neuron- specific autoimmune response, we generated mice expressing OVA as …

GP130-STAT3 regulates epithelial cell migration and is required for repair of the bronchiolar epithelium.

Following injury, bronchiolar cells undergo rapid squamous metaplasia, followed by proliferation and re-establishment of the complex …

Synthetic mimetics of the gp130 binding site for viral interleukin-6 as inhibitors of the vIL-6-gp130 interaction.

The transmembrane protein gp130 acts as the signal transducing receptor subunit for interleukin-6 type cytokines, including viral …

Susceptibility of four inbred mouse strains to a low-pathogenic isolate of Yersinia enterocolitica.

EUMORPHIA (European Union Mouse Research for Public Health and Industrial Application) is a research program involved in developing new …

Regulatory T cell-derived interleukin-10 limits inflammation at environmental interfaces.

The regulatory T (Treg) cells restrain immune responses through suppressor-function elaboration that is dependent upon expression of …

Mast cell-specific Cre/loxP-mediated recombination in vivo.

Mast cells are important effectors of type I allergy but also essential regulators of innate and adaptive immune responses. The aim of …

Differential molecular and anatomical basis for B cell migration into the peritoneal cavity and omental milky spots.

The constitutive migration of B cells from the circulation into the peritoneal cavity and back is essential for peritoneal B cell …

LMP1 signaling can replace CD40 signaling in B cells in vivo and has unique features of inducing class-switch recombination to IgG1.

The Epstein-Barr virus (EBV) protein LMP1 is considered to be a functional homologue of the CD40 receptor. However, in contrast to the …

Sphingosine-1 phosphate signaling regulates positioning of dendritic cells within the spleen.

A successful execution and balance of adaptive immune responses requires a controlled positioning and navigation of dendritic cells …

Contribution of interleukin-6/gp 130 signaling in hepatocytes to the inflammatory response in mice infected with Streptococcus pyogenes.

BACKGROUND: Sepsis and septic shock caused by gram-positive bacteria have become increasingly frequent clinical problems. These …

Serum response factor contributes selectively to lymphocyte development.

Serum response factor (SRF), is a crucial transcription factor for murine embryonic development and for the function of muscle cells …

Sequence and characterization of the Ig heavy chain constant and partial variable region of the mouse strain 129S1.

Although the entire mouse genome has been sequenced, there remain challenges concerning the elucidation of particular complex and …

The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens.

CD155, originally known as the cellular receptor for poliovirus, is the founding member of a subfamily of immunoglobulin-like adhesion …

Interleukin-10 derived from macrophages and/or neutrophils regulates the inflammatory response to LPS but not the response to CpG DNA.

Interleukin-10 (IL-10) is an important regulator of immune responses secreted by different cell types. We have previously shown that …

Dissecting the cytokine network.

Understanding the cytokine network is a very complex task. One way is the dissection of the network by the generation and analysis of …

VH replacement rescues progenitor B cells with two nonproductive VDJ alleles.

Inaccurate VDJ rearrangements generate a large number of progenitor (pro)-B cells with two nonproductive IgH alleles. Such cells lack …

Adult murine hematopoiesis can proceed without beta1 and beta7 integrins.

The function of alpha4beta1 and alpha4beta7 integrins in hematopoiesis is controversial. While some experimental evidence suggests a …

Terminal B cell differentiation is skewed by deregulated interleukin-6 secretion in beta2 integrin-deficient mice.

Absence of the common beta chain (CD18) of beta(2) integrins leads to leukocyte-adhesion deficiency type-1 (LAD1) in humans. Mice with …

A change of expression in the conserved signaling gene MKK7 is associated with a selective sweep in the western house mouse Mus musculus domesticus.

Changes in gene expression are known to occur between closely related species, but it is not yet clear how many of these are due to …

gp130 signaling in proopiomelanocortin neurons mediates the acute anorectic response to centrally applied ciliary neurotrophic factor.

Ciliary neurotrophic factor (CNTF) exerts anorectic effects by overcoming leptin resistance via activation of hypothalamic neurons. …

Nine fluorescence parameter analysis on a four-color fluorescence activated flow cytometer.

BACKGROUND: In many cases a frequent monitoring of blood is important, e.g. during an infection. Often the availability of blood is the …

Enhanced FTY720-mediated lymphocyte homing requires G alpha i signaling and depends on beta 2 and beta 7 integrin.

The immunomodulatory drug FTY720 interferes with sphingosine-1-phosphate (S1P) receptor signaling leading to lymphocyte retention in …

Virus free, cell-based assay for the quantification of murine type I interferons.

Interferons (IFNs) are potent biologically active proteins synthesized and secreted by somatic cells of all mammalian species. They …

Heterozygous deficiency of manganese superoxide dismutase results in severe lipid peroxidation and spontaneous apoptosis in murine myocardium in vivo.

To circumvent the early lethality of manganese superoxide dismutase (SOD2)-deficient mice, we have used a skin-specific strategy with …

VBASE2, an integrative V gene database.

The database VBASE2 provides germ-line sequences of human and mouse immunoglobulin variable (V) genes. It acts as an interconnecting …

Pre-B cell receptor expression is necessary for thymic stromal lymphopoietin responsiveness in the bone marrow but not in the liver environment.

IL-7 and thymic stromal lymphopoietin (TSLP) are two major cytokines controlling murine B cell development. IL-7 has been studied …

Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte- expressed protein.

Three mouse lines expressing Cre recombinase under the control of the human K14 promoter induced specific deletion of loxP flanked …

CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis.

In a CD18 hypomorphic polygenic PL/J mouse model, the severe reduction of CD18 (beta(2) integrin) to 2-16% of wild-type levels leads to …

The p53-dependent effects of macrophage migration inhibitory factor revealed by gene targeting.

Macrophage migration inhibitory factor (MIF) is a mediator of host immunity and functions as a high, upstream activator of cells within …

Thymic stromal-derived lymphopoietin distinguishes fetal from adult B cell development.

Deletions of interleukin 7 (IL-7) or its receptor components permit fetal but not adult B cell development in mice. Mice deficient in …

Lack of gp130 expression in hepatocytes promotes liver injury.

BACKGROUND & AIMS: Interleukin 6 (IL-6) contributes via its signal transducer gp130 to the acute phase response (APR) in …

Interleukin 6/gp130-dependent pathways are protective during chronic liver diseases.

The contribution of the acute phase inducer interleukin 6 (IL-6) in the pathogenesis of liver diseases is yet unclear. Our analysis …

IL-15 is an essential mediator of peripheral NK-cell homeostasis.

Several distinct classes of surface receptors can, on ligand binding, transmit signals that modulate the survival, proliferation, and …

Mice with neonatally induced inactivation of the vascular cell adhesion molecule-1 fail to control the parasite in Toxoplasma encephalitis.

Under various inflammatory conditions, cell adhesion molecules are up- regulated in the central nervous system (CNS) and may contribute …

Interleukin-6/glycoprotein 130-dependent pathways are protective during liver regeneration.

After tissue loss the liver has the unique capacity to restore its mass by hepatocyte proliferation. Interleukin-6 (IL6)-deficient mice …

Monoclonal antibody against beta7 integrins, but not beta7 deficiency, attenuates intestinal allograft rejection in mice.

BACKGROUND: beta7 integrins mediate homing and retention of lymphocytes to the normal and inflamed small bowel in a tissue-selective …

Beta7 integrins contribute to skin graft rejection.

BACKGROUND: Because integrins alpha4beta7 and alphaEbeta7 contribute to epidermotropism of T-cells during skin inflammation, we sought …

beta7 Integrin expression is not required for the localization of T cells to the intestine and colitis pathogenesis.

beta7 Integrins have been shown to have an important role in the localization of T cells to the intestine. Utilizing two different …

Integrin alpha 2-deficient mice develop normally, are fertile, but display partially defective platelet interaction with collagen.

The integrin alpha(2)-subunit was ablated in mice by targeted deletion of the ITGA2 gene. alpha(2)-Deficient animals develop normally, …

Neonatally induced inactivation of the vascular cell adhesion molecule 1 gene impairs B cell localization and T cell-dependent humoral immune response.

Vascular cellular adhesion molecule (VCAM)-1 is a membrane-bound cellular adhesion molecule that mediates adhesive interactions between …

Protective intestinal anti-rotavirus B cell immunity is dependent on alpha 4 beta 7 integrin expression but does not require IgA antibody production.

Rotavirus (RV) is the main cause of severe gastroenteritis in young children; protection has been correlated with intestinal Ab …

Role of STAT3 and PI 3-kinase/Akt in mediating the survival actions of cytokines on sensory neurons.

The binding of cytokines to the gp130 receptor activates the STAT3, MEK/MAPK, and PI3K/Akt signalling pathways. To assess the relative …

alpha(4)beta(7) independent pathway for CD8(+) T cell-mediated intestinal immunity to rotavirus.

Rotavirus (RV), which replicates exclusively in cells of the small intestine, is the most important cause of severe diarrhea in young …

Common cytokine receptor gamma chain (gammac)-deficient B cells persist in T cell-deficient gammac-mice and respond to a T-independent antigen.

Defects in the common cytokine receptor gamma chain (gammac) in man result in X-linked severe combined immunodeficiency disease …

Beta7 integrins contribute to demyelinating disease of the central nervous system.

A role for alpha4 integrins in different forms of the multiple sclerosis- like disease experimental autoimmune encephalomyelitis (EAE) …

Mice reconstituted with DNA polymerase beta-deficient fetal liver cells are able to mount a T cell-dependent immune response and mutate their Ig genes normally.

The ubiquitously expressed, error-prone DNA polymerase beta (polbeta) plays a role in base excision repair, and the involvement of this …

Chronic colitis in IL-10-/- mice: insufficient counter regulation of a Th1 response.

IL-10-deficient (IL-10-/-) mice, generated by a gene-targeted mutation, develop abnormal immune responses as a result of uncontrolled …

The role of beta7 integrins in CD8 T cell trafficking during an antiviral immune response.

The requirement of beta7 integrins for lymphocyte migration was examined during an ongoing immune response in vivo. Transgenic mice …

Loss of a gp130 cardiac muscle cell survival pathway is a critical event in the onset of heart failure during biomechanical stress.

Biomechanical stress is a major stimulus for cardiac hypertrophy and the transition to heart failure. By generating mice that harbor a …

Rearrangement and expression of immunoglobulin light chain genes can precede heavy chain expression during normal B cell development in mice.

In mouse mutants incapable of expressing mu chains, VkappaJkappa joints are detected in the CD43(+) B cell progenitors. In agreement …

IMGT, the international ImMunoGeneTics database.

IMGT, the international ImMunoGeneTics database (http://imgt.cnusc.fr:8104), is a high-quality integrated database specialising in …

Efficient lymphocyte migration across high endothelial venules of mouse Peyer's patches requires overlapping expression of L-selectin and beta7 integrin.

Lymphocyte migration into lymphoid organs is regulated by adhesion molecules including L-selectin and the beta7 integrins. L-selectin …

Postnatally induced inactivation of gp130 in mice results in neurological, cardiac, hematopoietic, immunological, hepatic, and pulmonary defects.

The pleiotrophic but overlapping functions of the cytokine family that includes interleukin (IL)-6, IL-11, leukemia inhibitory factor, …

L-selectin and beta7 integrin synergistically mediate lymphocyte migration to mesenteric lymph nodes.

Mesenteric lymph nodes (MLN) drain the gut where nutritive antigens and pathogens are encountered by lymphocytes of the gut-associated …

The roles of L-selectin, beta 7 integrins, and P-selectin in leukocyte rolling and adhesion in high endothelial venules of Peyer's patches.

Lymphocyte trafficking into Peyer's patches requires beta 7 integrins and L-selectin. Here, we use intravital microscopy to examine …

Regulated expression of gp130 and IL-6 receptor alpha chain in T cell maturation and activation.

The functional receptor for the inflammatory cytokine IL-6 is composed of the ligand binding IL-6 receptor alpha chain (IL-6R alpha) …

c-fos expression interferes with thymus development in transgenic mice.

To study the function of the proto-oncogene c-fos in hematopoietic tissues, transgenic mice were generated that express c-fos from the …

IL-10 regulates liver pathology in acute murine Schistosomiasis mansoni but is not required for immune down-modulation of chronic disease.

We have used IL-10 gene knockout mice (IL-10T) to examine the role of endogenous IL-10 in the down-modulation of hepatic granuloma …

IMGT, the International ImMunoGeneTics database.

IMGT, the international ImMunoGeneTics database, is an integrated database specialising in Immunoglobulins (Ig), T cell Receptors (TcR) …

IMGT, the international ImMunoGeneTics database: a new design for immunogenetics data access.

IMGT, the international ImMunoGeneTics database is an integrated database specializing in Immunoglobulins (Ig), T-cell receptors (TcR) …

Generation of Cre recombinase-specific monoclonal antibodies, able to characterize the pattern of Cre expression in cre-transgenic mouse strains.

Transgene-encoded Cre recombinase can target alteration of loxP-tagged genes to specific cell types and developmental stages in mice, …

Histological studies of gene-ablated mice support important functional roles for natural killer cells in the uterus during pregnancy.

Maternal lymphocytes having a large and granulated morphology accumulate at healthy implantation sites in normal mice. Insight into the …

A T cell clone which responds to interleukin 2 but not to interleukin 4.

The T cell clone CSP 2.1 is stimulated into proliferation by interleukin (IL) 2 but not IL 4, whereas many T cells respond to both. …

Interleukin-4 protects against a genetically linked lupus-like autoimmune syndrome.

Interleukin-4 (IL-4) provides support for humoral immune responses through upregulation of T helper (Th) type 2 cell differentiation, …

IMGT, the international ImMunoGeneTics database.

IMGT, the international ImMunoGeneTics database, is an integrated database specializing in immunoglobulins, T-cell receptors (TcR) and …

Plasmodium chabaudi chabaudi: differential susceptibility of gene- targeted mice deficient in IL-10 to an erythrocytic-stage infection.

Female and male mice deficient in IL-10 production by targeted disruption of the IL-10 gene were infected with Plasmodium chabaudi …

Interleukin (IL)-4-independent immunoglobulin class switch to immunoglobulin (Ig)E in the mouse.

Immunoglobulin (Ig) class switching in B cells is regulated by stimuli transduced by cytokines and cell-cell contact. Among these …

Impaired immunosuppressive response to ultraviolet radiation in interleukin-10-deficient mice.

Exposure to mid-range ultraviolet radiation (UVR) 280-320 nm, ultraviolet B (UVB) radiation] inhibits the acquisition of delayed-type …

Bypass of lethality with mosaic mice generated by Cre-loxP-mediated recombination.

BACKGROUND: The analysis of gene function based on the generation of mutant mice by homologous recombination in embryonic stem cells is …

Enterocolitis and colon cancer in interleukin-10-deficient mice are associated with aberrant cytokine production and CD4(+) TH1-like responses.

We have characterized the progressive stages of chronic intestinal inflammation that develops spontaneously in specific pathogen-free …

Critical role for beta7 integrins in formation of the gut-associated lymphoid tissue.

Immune defence against pathogens entering the gut is accomplished by lymphocytes in the gut-associated lymphoid tissue (GALT), a major …

T helper cell 1-type CD4+ T cells, but not B cells, mediate colitis in interleukin 10-deficient mice.

Mice rendered deficient in the production of interleukin 10 (IL-10-/-) develop a chronic inflammatory bowel disease (IBD) that …

Surrogate light chain expression is required to establish immunoglobulin heavy chain allelic exclusion during early B cell development.

Allelic exclusion at the IgH locus was examined in B lineage cells of wild-type mice and mice unable to express the surrogate light …

Leishmania promastigotes selectively inhibit interleukin 12 induction in bone marrow-derived macrophages from susceptible and resistant mice.

Leishmania major promastigotes were found to avoid activation of mouse bone marrow-derived macrophages (BMM0) in vitro for production …

Interleukin-10 is a central regulator of the response to LPS in murine models of endotoxic shock and the Shwartzman reaction but not endotoxin tolerance.

Previous studies in vivo have shown that IL-10 infusion can prevent lethal endotoxic shock. Mice deficient in the production of IL-10 …

A role for CD5 in TCR-mediated signal transduction and thymocyte selection.

CD5 is a transmembrane protein that is expressed on the surface of T cells and a subset of B cells. The absence of CD5 rendered …

Interleukin 10 but not interleukin 4 is a natural suppressant of cutaneous inflammatory responses.

We have examined the role of endogenously produced interleukin (IL) 4 and IL-10 in the regulation of inflammatory and immune reactions …

Mouse anti-mouse IgD monoclonal antibodies generated in IgD-deficient mice.

The production of high-affinity monoclonal antibodies (mABs) is generally restricted to antigens recognised as foreign by the immune …

Altered immune responses in mice lacking inducible nitric oxide synthase.

Nitric oxide (NO) is important in many biological functions. It is generated from L-arginine by the enzyme NO synthase (NOS). The …

Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4.

Antiviral immune responses of mice lacking interleukin-2 (IL-2) or IL-4 or both IL-2 and IL-4 (IL-2/4) were compared by using different …

Lymphoid development in mice with a targeted deletion of the interleukin 2 receptor gamma chain.

The interleukin 2 receptor gamma chain (IL-2R gamma) is a component of the receptors for IL-2, IL-4, IL-7, and IL-15. Mutations in …

Interleukin 10 inhibits interleukin 6 production and acute phase response in rheumatoid arthritis.

To evaluate the effect of interleukin 10 (IL-10) on acute phase response, we determined serum levels of IL-10, interleukin 6 (IL-6), …

Cre-loxP-mediated gene replacement: a mouse strain producing humanized antibodies.

BACKGROUND: The bacteriophage-derived Cre-loxP recombination system operates efficiently in mammalian cells. This system is …

Lymphocyte populations and immune responses in CD5-deficient mice.

The CD5 antigen is expressed at a high level on T cells from early on in thymocyte ontogeny and continues to be expressed on the …

Analysis of the B-cell progenitor compartment at the level of single cells.

BACKGROUND: During B-cell development in the mouse, the VH, DH and JH elements of the immunoglobulin heavy chain (IgH) locus are …

Induction of interleukin 4 (IL-4) expression in T helper (Th) cells is not dependent on IL-4 from non-Th cells.

Interleukin 4 (IL-4) is essential for the induction of immunoglobulin E (IgE) responses in mice. Recent in vitro studies have suggested …

Development and proliferation of lymphocytes in mice deficient for both interleukins-2 and -4.

Interleukin (IL)-2 and IL-4 are considered as important regulators of growth and differentiation of lymphocytes. We report that in mice …

Immunoglobulin heavy and light chain genes rearrange independently at early stages of B cell development.

The compartment of mouse B cell progenitors can be resolved into five developmentally related fractions by multicolor flow cytometry. …

Interleukin-4 transgenic mice of resistant background are susceptible to Leishmania major infection.

The outcome of cutaneous leishmaniasis is dependent on the balance of Th1 and Th2 cells. In the murine model, Th1 cells are …

Interleukin-10-deficient mice develop chronic enterocolitis.

Interleukin-10 (IL-10) affects the growth and differentiation of many hemopoietic cells in vitro; in particular, it is a potent …

Long-term consequences of interleukin-6 overexpression in transgenic mice.

With the aim of using interleukin-6 (IL-6)-inducible promoters to express transgenes, we investigated the long-term consequences of …

T helper cell membranes promote IL-4-independent expression of germ-line C gamma 1 transcripts in B cells.

Studies using plasma membranes from activated Th cell clones (Th membranes) to stimulate B cells have shown that both a …

A critical role of lambda 5 protein in B cell development.

The lambda 5 gene is a homolog of immunoglobulin J lambda-C lambda genes, expressed specifically in immature B-lineage cells. Lambda …

Analysis of cytokine mRNA levels in interleukin-4-transgenic mice by quantitative polymerase chain reaction.

Interleukin (IL)-4-transgenic mice were used as a model system to study the consequences of low levels of IL-4 expression for the …

Generation of long-lived B cells in germ-free mice.

In the present study we have addressed the question of whether the composition of the peripheral B cell pool is similar in mice raised …

Most peripheral B cells in mice are ligand selected.

Using amplified cDNA and genomic libraries, we have analyzed the VH gene repertoire of pre-B cells and various B cell subsets of …

Tumor suppression after tumor cell-targeted tumor necrosis factor alpha gene transfer.

The tumor necrosis factor alpha (TNF-alpha) gene was introduced by retroviral gene transfer into the TNF-alpha-insensitive tumor cell …

Major histocompatibility complex class II hyperexpression on B cells in interleukin 4-transgenic mice does not lead to B cell proliferation and hypergammaglobulinemia.

The murine interleukin 4 (IL4)-encoding cDNA expressed under the control of the immunoglobulin heavy chain enhancer/promoter was …

Generation and analysis of interleukin-4 deficient mice.

Interleukin-4 (IL-4) promotes the growth and differentiation of many hematopoietic cells in vitro; in particular, it directs the …

Retroviral interleukin 4 gene transfer into an interleukin 4-dependent cell line results in autocrine growth but not in tumorigenicity.

A retroviral vector has been constructed containing the mouse interleukin 4 (IL 4) gene under the transcriptional control of the …

High gradient magnetic cell separation with MACS.

A flexible, fast and simple magnetic cell sorting system for separation of large numbers of cells according to specific cell surface …

Membrane-bound IgM obstructs B cell development in transgenic mice.

Rearranged immunoglobulin genes encoding either the secreted (microsecond) or membrane (micron) form of IgM were introduced into the …

Class switch recombination is IgG1 specific on active and inactive IgH loci of IgG1-secreting B-cell blasts.

Mouse B lymphocytes can be activated polyclonally by bacterial lipopolysaccharide (LPS) to secrete Ig and perform Ig class switch. In …

Cell-cooling in flow cytometry by Peltier elements.

We have built a cooling device for cell suspensions in flow cytometry that makes use of the Peltier effect (Barnard RD, Thermo …

A new V gene expressed in lambda-2 light chains of the mouse.

We have partially sequenced the light chain variable regions expressed in three IgM-producing hybridomas generated from newborn mice or …

Lymphokines regulate immunoglobulin isotype expression in an antigen- specific immune response.

The control of immunoglobulin class switching appears to involve T cell- derived lymphokines. Such lymphokines have been shown to …

Control of immunoglobulin class switch recombination.

The comparative analysis of Ig class switch recombination in a priori IgG/IgA-expressing myelomas and hybridomas, in switch variants …

Signal requirements for growth and differentiation of activated murine B lymphocytes.

Mouse B lymphocytes were stimulated at high cell concentrations with goat anti-IgM antibodies, which leads to the induction of B cell …

Heterogeneous and monoclonal helper T cells induce similar anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibody populations in the primary adoptive response. I. Isotype distribution.

In the response to NP-lipopolysaccharide or NP-Ficoll predominantly anti- NP antibodies of the IgM class are produced in mice with …

Mycoplasma contamination in human tumor cell lines: effect on interferon induction and susceptibility to natural killing.

Cleaning of human tumor cell lines from arginine-dependent nonfermentative Mycoplasma orale 1 (MO1) by a recently developed technique …